Genetic interaction mapping and exon-resolution functional genomics with a hybrid Cas9-Cas12a platform
Gonatopoulos-Pournatzis T, Aregger M, Brown KR, Farhangmehr S, Braunschweig U, Ward HN, Ha KCH, Weiss A, Bilmann M, Durbic T, Myers Cl, Blencowe BJ, Moffat J.  Nature Biotechnology; in press. DOI: 10.1038/s41587-020-0437-z


Library Files

human hgRNA optimization library  | mouse hgRNA optimization library  | human exon deletion library  | human dual targeting paralog libary

NovaSeq Raw Read Counts

mouse optimization screen (merged) | human optimization screen (merged) | human dual targeting paralog screen | exon deletion


A convolutional neural network for Cas12a guide scoring (GitHub)

CHyMErA Scoring

Code for CHyMErA dual-targeting, paralog and exon deletion scoring (GitHub)
Code for CHyMErA exon-deletion scoring (GitHub)